High Speed Ion-Exchange Chromatography of Amino Acids
نویسندگان
چکیده
منابع مشابه
High-speed Liquid Chromatography of Sugar Acids on An- Ion-exchange Resins
Mixtures of aldonic and aldaric acids are difficult to analyse. Aldonic acids can be separated in a satisfactory way on anion-exchange resins with a solution of sodium chloride as eluent. Under these circumstances, however, aldaric acids show unacceptably long elution times and peak broadening. The ability of aldaric acids to form non-adsorbable complexes with magnesium ions lowers their elutio...
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Chromatographic separations of amino acids on ion exchange resins may be performed on a preparative scale either by elution (24) or by displacement (5-12). Some of the exceptional resolving power demonstrated in analytical separations by elution techniques (13, 14) disappears when larger columns with relatively higher loads are used (2). Displacement chromatography allows large quantities of so...
متن کاملIon-exchange chromatography of amino acids in sweat collected from healthy subjects during sauna bathing.
Amino acids were measured by ion-exchange liquid chromatography in sweat samples obtained from 22 healthy men during sauna bathing. Amino acids were also measured in plasma (collected before breakfast) and urine (24-h collections) from the same subjects. Serine, ornithine, citrulline, and aspartic acid were present in sweat in mean concentrations >10-fold their mean concentrations in plasma. In...
متن کاملPreparative isolation of amino acids by carrier displacement chromatography on ion exchange resins.
Chromatographic separations of amino acids on ion exchange resins may be performed on a preparative scale either by elution (24) or by displacement (5-12). Some of the exceptional resolving power demonstrated in analytical separations by elution techniques (13, 14) disappears when larger columns with relatively higher loads are used (2). Displacement chromatography allows large quantities of so...
متن کاملNotes on the determination of amino acids by ion-exchange chromatography.
Dowex 50. The resin, kindly supplied by Dow Chemical International Ltd., Midland, Michigan, U.S.A., was stated to be of nominal 12% cross-linking and 200-400 mesh. Sodium phosphate buffer pH 6-8, 0.1M. This buffer was adjusted to pH 6-75 in order to delay slightly the emergence of the histidine peak. At the lower pH, histidine emerges separated from and almost mid-way between hydroxylysine and ...
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ژورنال
عنوان ژورنال: NIPPON KAGAKU KAISHI
سال: 1976
ISSN: 0369-4577,2185-0925
DOI: 10.1246/nikkashi.1976.623